Device
Toggle

Part:BBa_K415300:Design

Designed by: Leanna Morinishi, Grant Robinson, Andrew Yang, Paul Muir, Shirley Galbiati, Shawn Pan, Jason Stevens, Lauren McGough, Deepak Mishra, Ron Weiss   Group: iGEM10_MIT   (2010-10-23)


Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used PCR to biobrick this sequence from its original form in an Amp plasmid with SC101 origin. We had to transfer into pSB1C3 to submit it to the registry.


Source

Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.

References